Time course | Cytokines | Accession number | 4 hours pi | 1 day pi | 7 days pi |
|---|
3 time points | MIP-1-α (CCL3) | M23452 | ++ (++) | + (+) | + (++) |
|  | MIP-1-β (CCL4) | J04130 | + (++) | + (+) | + (++) |
2 time points | IL-8 (CXCL8) | Y00787 | - | + | + |
1 time point | SCYA-1 (CCL1) | M57502 | +++ | - | - |
| Â | BCA-1 (CXC) | AJ002211 | - | + | - |
| Â | MIG (CXCL9) | X72755 | - | ++ | - |
| Â | IP-10 (CXCL10) | X02530 | - | ++ | - |
| Â | MCP-1 (CCL2) | M24545 | - | - | + |
|  | MIP-2-α (CXCL2) | X53799 | - | - | + |
- The table shows mRNA transcripts encoding for chemokines as found to be expressed by microarray in Chlamydia trachomatis-infected human monocytes over a time course of up to 7 days. Microarray ratios of MIP-1-α and MIP-1-β were confirmed by quantitative real-time reverse transcription-polymerase chain reaction (noted in parentheses) (see Figures 1–3). Fold of expression for microarray as well as for reverse transcription-polymerase chain reaction data is indicated by '-' (less than 2), '+' (more than or equal to 2 and less than 10), '++' (more than or equal to 10 and less than 100), and '+++' (more than or equal to 100). Gene expression was studied at three different time points: 4 hours pi indicates the very early phase of active infection, whereas day 1 corresponds to a transition (that is, the beginning of growth arrest) and day 7 represents persistent infection. BCA, B-cell-attracting chemokine; IL, interleukin; IP, interferon inducible protein; MCP, monocyte chemotactic protein; MIG, monokine induced by gamma-interferon; MIP, macrophage inflammatory protein; SCYA, small inducible cytokine.
