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Table 2 mRNA transcript expression in microarray

From: Expression of inflammatory host genes in Chlamydia trachomatis-infected human monocytes

Time course Cytokines Accession number 4 hours pi 1 day pi 7 days pi
3 time points MIP-1-α (CCL3) M23452 ++ (++) + (+) + (++)
  MIP-1-β (CCL4) J04130 + (++) + (+) + (++)
2 time points IL-8 (CXCL8) Y00787 - + +
1 time point SCYA-1 (CCL1) M57502 +++ - -
  BCA-1 (CXC) AJ002211 - + -
  MIG (CXCL9) X72755 - ++ -
  IP-10 (CXCL10) X02530 - ++ -
  MCP-1 (CCL2) M24545 - - +
  MIP-2-α (CXCL2) X53799 - - +
  1. The table shows mRNA transcripts encoding for chemokines as found to be expressed by microarray in Chlamydia trachomatis-infected human monocytes over a time course of up to 7 days. Microarray ratios of MIP-1-α and MIP-1-β were confirmed by quantitative real-time reverse transcription-polymerase chain reaction (noted in parentheses) (see Figures 1–3). Fold of expression for microarray as well as for reverse transcription-polymerase chain reaction data is indicated by '-' (less than 2), '+' (more than or equal to 2 and less than 10), '++' (more than or equal to 10 and less than 100), and '+++' (more than or equal to 100). Gene expression was studied at three different time points: 4 hours pi indicates the very early phase of active infection, whereas day 1 corresponds to a transition (that is, the beginning of growth arrest) and day 7 represents persistent infection. BCA, B-cell-attracting chemokine; IL, interleukin; IP, interferon inducible protein; MCP, monocyte chemotactic protein; MIG, monokine induced by gamma-interferon; MIP, macrophage inflammatory protein; SCYA, small inducible cytokine.