Table 1 Primers used in the present study
Primer | Sequence |
|---|---|
β-actin | Forward 5'-3' AAGCCACCCCACTTCT-CTCTAA |
Reverse 5'-3' AATGCTATCACCTCCCCTGTGT | |
COL1A2 | Forward 5'-3'TTGCCCAAAGTT-GTCCTCTTCT |
Reverse 5'-3' AGCTTCTGTGGAACCATGGAA | |
COL2A1 | Forward 5'-3' CTGCAAAATAAAATCTCGGTGTTCT |
Reverse 5'-3' GGGCATTTGACTCACACCAGT | |
HIF-1α | Forward 5-3' GTAGTTGTGGAAGT-TTATGCTAATATTGTGT |
Reverse 5'-3' CTTGTTTACAGTCTGCTCA-AAATATCTT | |
P4Hα(I) | Forward 5'-3' GCAGGGTGGTAATATTGGCATT |
Reverse 5'-3' AAATCAATTCCCTCATCACTGAAAG, | |
P4Hα(II) | Forward 5'-3'TTAGCTGTCTAGCGCCTAGCAA |
Reverse 5'-3' TTTGGTTCACTGAAACA-TCTCACA | |
P4Hα(III) | Forward 5'-3' CTCAACAGTCTCAGGTTCGATCA |
Reverse 5'-3' TTCTTGGTCCCTGTGGTCAAG | |
PHD2 | Forward 5'-3'TGGCC-TATATGTGTTTAATCCTGGTT |
Reverse 5'-3'TGTTTTACAGCTGGTTAATGTG-TTGA | |
SOX9 | Forward 5'-3'CTTTGGTTTGTGTTCGTGTTTTG |
Reverse 5'-3'AGAGAAAGAAAAAGGGAAAGGTAAGTTT |