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Table 2 PCR primer and probe sequences and efficiencies

From: Catabolic cytokine expression in degenerate and herniated human intervertebral discs: IL-1β and TNFα expression profile

Target Forward primer Probe Reverse primer Efficiency (percent)
18s PDAR PDAR PDAR 99.65
IL-1β 5' CGG CCA CAT TTG GTT CTA AGA 3' 5' ACC CTC TGT CAT TCG CTC CCA CA 3' 5' AGG GAA GCG GTT GCT CAT C 3' 90.5
TNF α 5' TGG TGG TCT TGT TGC TTA AAG TTC 3' 5' TCC CCT GCC CCA ATC CCT TTA TTA CCC G 3' 5' CGA ACA TCC AAC CTT CCC AAA C 3' 90.1
IL-1 RI 5' ATT TCT GGC TTC TAG TCT GGT GTT C 3' 5' ACT TGA TTT CAG GTG AAT AAC GGT CCC C 3' 5' AAC GTG CCA GTG TGG AGT GA 3' 98.5
TNF RI 5' CCT GGC CCC AAA CCC AAG 3' 5' TTC AGT CCC ACT CCA GGC TTC ACC C 3' 5' GTA TAG GTG GAG CTG GAG GTG 3' 93.8
  1. RI, receptor type I; TNF, tumour necrosis factor. PDAR, Pre-developed assay reagents.