Figure 1
TauCl differentially inhibits the expression of MMPs in IL-β-stimulated RA FLSs. The expressions of the collagenases (matrix metalloproteinase [MMP]-1 and MMP-13) and the gelatinases (MMP-2 and MMP-9) were determined by (a) ELISA analysis, (b) real time PCR and (c) semi-quantitative RNA analysis. Synovial cells (5 × 105 cells/60 mm dish/2 ml serum-free media) were treated with taurine chloramine (TauCl) 30 min before 24 hours of IL-1β (10 ng/ml) stimulation for MMP protein analysis by ELISA. Cells (2.5 × 105 cells/60 mm dish/2 ml serum-free media) were treated with TauCl 30 min before 6 hours of stimulation with IL-1β (10 ng/ml) for RNA level analysis. IL-1β stimulated the expression of the MMP-1 and MMP-13 genes, but it did not affect the expression of MMP-2 or MMP-9. TauCl differentially inhibited the expressions of MMP-1 and MMP-13. Experiments were performed in duplicate with cells from three patients. Values are expressed as means ± standard deviation. *P < 0.01 versus control group (no IL-1β); #P < 0.05 and ##P < 0.01 versus IL-1β treatment group without TauCl. FLS, fibroblast-like synoviocyte; PBS, phosphate-buffered saline; RA, rheumatoid arthritis.