- Meeting abstract
Evidence for an antigen-driven immune response in the chronically inflamed synovium
Arthritis Research & Therapy volume 1, Article number: S09 (1999)
Recently it has been shown that proinflammatory cytokines, like TNF-alpha and lymphotoxin play a crucial role in the organogenesis of the lymphoid tissue. Thus, during chronic inflammation, in the affected tissue, these cytokines may promote the development of a micro-environment which supports a local immune response. In patients with rheumatoid arthritis large lymphocytic infiltrates are often seen in the synovial tissue. These cell clusters have a characteristic arrangement of T, B and plasma cells. Proliferating B cells are found only centrally in a network of follicular dendritic cells. Using micro-manipulation CD20+ B cells and plasma cells were isolated separately from different parts of single infiltrates. DNA was extracted and the VH/VL gene repertoires determined. The data show that within the network of follicular dendritic cells there is an oligoclonal expansion of B cells. During proliferation V-gene variants are generated by the hypermutation mechanism. The pattern of somatic mutations suggests that both naive and memory B cells become activated in the synovial tissue. Within single infiltrates we did not find identical rearrangements between CD20+ B and plasma cells. Nevertheless, the finding of clonally related plasma cells suggests that these cells underwent terminal differentiation in the synovial tissue. The analysis of individual B cells recovered from synovial tissue opens a new way to determine the specificity of those cells which take part in the local immune reaction.
Berek C, Kim H-J: B-cell activation and development within chronically inflamed synovium in rheumatoid and reactive arthritis. Sem Immunol. 1997, 9: 261-268. 10.1006/smim.1997.0076.
Fu Y-X, Chaplin DD: Development and maturation of secondary lymphoid organs. Annu Rev Immunol. 1999, 17: 399-433. 10.1146/annurev.immunol.17.1.399.
Kim H-J, Krenn V, Steinhauser G, Berek C: Plasma cell development in synovial germinal centers in patients with rheumatoid and reactive arthritis. J Immunol. 1999, 162: 3053-3062.
Randen I, Mellbye J, Forre O, Natvig JB: The identification of germinal centres and follicular dendritic networks in rheumatoid synovial tissue. Scand J Immuol. 1995, 41: 481-486.
Schröder AE, Greiner A, Seyfert C, Berek C: Differentiation of B cells in the nonlymphoid tissue of the synovial membrane of patients with rheumatoid arthritis. Proc Natl Acad Sci USA. 1996, 93: 221-225. 10.1073/pnas.93.1.221.
About this article
Cite this article
Bearek, C., Kim, HJ. Evidence for an antigen-driven immune response in the chronically inflamed synovium. Arthritis Res Ther 1 (Suppl 1), S09 (1999). https://doi.org/10.1186/ar23