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Table 1 Effect of anti-CD47/IAP, anti-TSP-1, and anti-SIRPα on mechanically induced changes in human articular chondrocyte membrane potential

From: CD47 associates with alpha 5 integrin and regulates responses of human articular chondrocytes to mechanical stimulation in an in vitromodel

   Membrane potential (-mV) (mean ± SEM)  
Sample Reagent Resting Reagent alone Reagent + 0.33 Hz Percentage change
Normal chondrocytes Nil 25.0 ± 0.4 - 30.2 ± 0.6 +21a
  Bric 126 26.2 ± 0.8 27.2 ± 0.4 27.6 ± 1.2 +2b
  B6H12 25.8 ± 2.2 26.3 ± 1.9 17.1 ± 1.0 -35a
Osteoarthritic chondrocytes Nil 30.0 ± 1.9 - 17.4 ± 1.3 -42a
  Bric 126 28.0 ± 1.6 28.0 ± 2.1 27.4 ± 1.8 -2b
  Anti-TSP-1 29.6 ± 1.2 29.0 ± 0.8 29.8 ± 1.6 +3b
  SE5A5 29.2 ± 1.0 29.0 ± 1.1 23.2 ± 0.8 -20a
  1. Membrane potentials of chondrocytes were measured at rest in the absence or presence of the appropriate antibody and after 20 minutes of mechanical stimulation at 0.33 Hz. Bric 126 is a function-blocking anti-CD47/IAP antibody. B6H12 is an anti-CD47/IAP antibody that has partial agonist activity. SE5A5 is an anti-SIRPα antibody. N = 5. ap < 0.001; bnot significant. IAP, integrin-associated protein; SEM, standard error of the mean; SIRPα, signal-regulatory protein-alpha; TSP-1, thrombospondin-1.