Skip to main content

Table 2 Primers used for real-time PCR and product length

From: Anti-inflammatory effect of antidiabetic thiazolidinediones prevents bone resorption rather than cartilage changes in experimental polyarthritis

Gene encoding Primer sequence Size (base pairs) Tm (°C)
RP29 Sense: 5'-AAGATGGGTCACCAGCAGCTCTACTG-3' 67 59
  Antisense: 5'-AGACGCGGCAAGAGCGAGAA-3'   
Aggrecan Sense: 5'-ACACCCCTACCCTTGCTTCT-3' 124 58
  Antisense: 5'-AAAGTGTCCAAGGCATCCAC-3'   
PPAR-α Sense: 5'-GATGACCTGGAAAGTCCCTT-3' 59 56
  Antisense: 5'-CTTGAATGTTTCCCATCTCTT-3'   
PPAR-γ Sense: 5'-ATGGGTGAAACTCTGGGAGAT-3' 92 56
  Antisense: 5'-GGTAATTTCTTGTGAAGTGCT-3'   
Adiponectin Sense: 5'-AATCCTGCCCAGTCATGAAG-3' 433 58
  Antisense: 5'-TCTCCAGGAGTGCCATCTCT-3'   
ACO Sense: 5'-CCAATCACGCAATAGTTCTGG-3' 362 57
  Antisense: 5'-CGCTGTATCGTATGGCGAT-3'   
  1. PPAR, peroxisome proliferator-activated receptor; ACO, acyl-CoenzymeA oxidase; Tm, melting temperature.