Resting CD4+effector memory T cells are precursors of bystander-activated effectors: a surrogate model of rheumatoid arthritis synovial T-cell function

Table 3 Modulatory effect on spontaneous TNF-α production following blockade of cell surface ligands in RA MNC cultures

RA donor	Anti-CD69		Anti-CD18		Anti-CD49d
	Inhibition (%)	P	Inhibition (%)	P	Inhibition (%)	P
1	17	<0.01	0	NS	0	NS
2	23	<0.05	22	<0.01	36	<0.01
3	22	NS	26	NS	23	NS
4	ND	NS	51	<0.01	0	NS

Rheumatoid arthritis (RA) synovial mononuclear cell (MNC) cells were cultured in triplicate with anti-CD69, anti-CD18, or anti-CD49d antibodies at a final concentration of 5 μg/ml for 24 hours at 37°C, as described in Materials and methods. Spontaneous tumour necrosis factor (TNF)-α production was measured in the supernatants by ELISA and results expressed as percentage inhibition relative to the control wells (cells only). Significance (P value) was calculated using one-way analysis of variance with post hoc Bonferroni correction.

ISSN: 1478-6362