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Table 3 Modulatory effect on spontaneous TNF-α production following blockade of cell surface ligands in RA MNC cultures

From: Resting CD4+effector memory T cells are precursors of bystander-activated effectors: a surrogate model of rheumatoid arthritis synovial T-cell function

RA donor Anti-CD69 Anti-CD18 Anti-CD49d
  Inhibition (%) P Inhibition (%) P Inhibition (%) P
1 17 <0.01 0 NS 0 NS
2 23 <0.05 22 <0.01 36 <0.01
3 22 NS 26 NS 23 NS
4 ND NS 51 <0.01 0 NS
  1. Rheumatoid arthritis (RA) synovial mononuclear cell (MNC) cells were cultured in triplicate with anti-CD69, anti-CD18, or anti-CD49d antibodies at a final concentration of 5 μg/ml for 24 hours at 37°C, as described in Materials and methods. Spontaneous tumour necrosis factor (TNF)-α production was measured in the supernatants by ELISA and results expressed as percentage inhibition relative to the control wells (cells only). Significance (P value) was calculated using one-way analysis of variance with post hoc Bonferroni correction.