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Figure 7 | Arthritis Research & Therapy

Figure 7

From: TNF inhibits production of stromal cell-derived factor 1 by bone stromal cells and increases osteoclast precursor mobilization from bone marrow to peripheral blood

Figure 7

No changes in TNF-induced osteoclast precursor release from Cxcr4 f/f / CD11b+/Cre conditional knockout mice bone marrow. (a) Bone marrow cells from 2-month-old CXCR4 CKO and control mice were double stained with FITC-anti-CD11b and allophycocyanin (APC)–anti-CXCR4 antibodies, and CD11b+ cells were gated. Intensity of CXCR4+ stained CD11b+ cells shown. Black line, CXCR4 CKO cells; red line, control cells. (b) Murine TNF (0.5 μg/injection, 4 times/day × 3 days, intraperitoneally) or PBS was injected into 2-month-old CXCR4 CKO mice and control mice (n = 2 per group). Two hours after the last TNF injection, the peripheral blood was harvested and double stained with APC–anti-CD11b and Phycoerythrin-anti-Gr1 antibodies. The percentage of CD11b+/Gr-1-/lo osteoclast precursors (OCPs) were determined. Values from individual mice are plotted. bp, base pairs; WT, wild-type.

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Arthritis Research & Therapy

ISSN: 1478-6362

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