Figure 3

Effects of human HSP70 and lipopolysaccharide. The effects of human heat shock protein (HSP)70 on proinflammatory mediator secretion in fibroblast-like synoviocytes (FLSs) are not due to contaminating lipopolysaccharide (LPS). Rheumatoid arthritis (RA) FLSs were incubated with untreated, polymyxin B (PMB; 10 μg/ml)-treated, or boiling(100°C, 30 minutes)-treated human heat shock protein 70 (HSP70;1 μg/ml) or lipopolysaccharide (LPS; 100 ng/ml) for 1 hour, washed and then exposed to tumour necrosis factor (TNF)-α (20 ng/ml). The supernatants were harvested after 24 hours, and (a) IL-6, (b) IL-8 and (c) monocyte chemoattractant protein (MCP)-1 concentrations were determined using ELISA. Data are presented as means ± standard deviation of three independent experiments. *P < 0.05 versus TNF-α alone.