Role of hirudin, factor VII inhibitor and ancrod in soluble tissue factor-induced footpad inflammation. (a) Wildtype mice treated with PEG-hirudin (n = 5) or with ASIS (active-site inhibited activated factor VII, n = 5), or untreated mice (n = 7), were injected with 1 μg soluble tissue factor. Results from all treated groups were significantly reduced (P < 0.05 by t test) compared with the control group. (b) Wildtype mice were treated with ancrod (n = 7) or with phosphate-buffered saline (n = 7) and then injected with 1 μg soluble tissue factor. Results from ancrod-treated mice are significantly different from the control group, at all time points (P < 0.05 by t test).