Table 1 Profile of antibodies used in 3D immunohistological and CD marker studies
Antibody | Source | Dilution |
|---|---|---|
Type I collagen | Biodesign International (Kennebunk, ME) | 20 μg/ml |
Type II collagen | Biodesign International (Kennebunk, ME) | 20 μg/ml |
Decorin | R&D Systems, (Minneapolis, MN) | 25 μg/ml |
Keratin sulphate | Seikagaku Corporation, (Tokyo, Japan) | 5 μg/ml |
Chondroitin sulphate | ICN Biomedicals, (Costa Mesa, CA) | 20 μg/ml |
CD29 | Lab Vision Corporation (Fremont, CA) | 200 μg/ml |
CD34 | DakoCytomation (Carpinteria, CA) | 50 μg/ml |
CD45 | DakoCytomation, (Carpinteria, CA) | 350 μg/ml |
CD105 | Lab Vision Corporation (Fremont, CA) | 200 μg/ml |