Figure 3
The functional analysis of dendritic cells (DCs) and regulatory T cells (Tregs) from RP105-deficient mice. (a) The splenic DCs from type II collagen (CII)-immunized RP105+/+ or RP105-/- mice were mixed with pooled adherent cells removed lymph node cells (LNCs) from RP105+/- mice, as described in Materials and methods. The cells were stimulated with denatured CII for 2 days and interferon-gamma (IFN-γ) production was measured. Values are mean ± standard error. The DCs from RP105-/- mice induced higher IFN-γ production from LNCs than did the DCs from RP105+/+ at 50 × 104 cells. The summary of five experiments, using three mice per group, is shown (*P < 0.05, Mann-Whitney U test). (b) The Tregs (1 × 105) purified from the spleens of CII-immunized RP105+/+ and RP105-/- mice were mixed with pooled LNCs (1 × 106) from RP105+/- mice, which were immunized with CII 14 days before. The cells were stimulated with denatured CII (20 μg/mL) or anti-CD3 antibody (1 μg/mL) for 2 days and IFN-γ production was measured. The percentage suppression (100 × [IFN-γ without Tregs – IFN-γ with Tregs]/IFN-γ without Tregs) is shown. Values are mean ± standard error of the six experiments, using three mice per group (*P < 0.05, Wilcoxon matched pairs test).