Figure 3

Some T cells that expand in response to huCollp263–271 are spontaneously enriched in synovial fluid. (a) BV-BJ spectra of three rearrangements were obtained from PBMCs of patient OE cultured in the absence (control) or presence of huCollp263–271, or from cells harvested from inflamed knee joint. (b) CDR3 sequences of TCRs carrying BV1-BJ2.6 obtained from collagen peptide-stimulated PBMCs or inflammatory synovial fluid from patient OE. A box indicates that CDR3 sequences of the expected length that are present in multiple copies in both samples. huCollp261–273, human collagen peptide 261–273; PBMC, peripheral blood mononuclear cell; TCR, T-cell receptor.