Figure 2

Matrix metalloproteinase-1 (MMP-1) and MMP-13 mRNA production is specifically inhibited by rosiglitazone treatment. SW-1353 cells were incubated with 50 nM rosiglitazone for 24 hours followed by 1 ng/mL interleukin-1-beta (IL-1β) treatment for an additional 24 hours. Total RNA was harvested, and MMP mRNA levels were quantified using real-time reverse transcription-polymerase chain reaction. Y values are given as molecules of MMP per molecule of GAPDH (glyceraldehyde 3-phosphate dehydrogenase) mRNA. P values were calculated for the difference from the IL-1β sample using the Student t test (*P < 0.05, **P < 0.005). NoTx, no treatment; Rosi, rosiglitazone.