Dissection of a locus on mouse chromosome 5 reveals arthritis promoting and inhibitory genes

Table 3 Anti-collagen type II antibody responses in C2, C3, C4, and C5 congenic mice

Congenic fragment^a	Day after immunisation	Antibody isotype	a/a^b	a/b^c	b/b^d	p-value^e
C2	14	IgG1		65^f ± 7^g	115 ± 13	0.0006
		IgG2c		80 ± 8	114 ± 12	0.0172
		IgG3		97 ± 12	176 ± 25	0.0017
		IgM		113 ± 9	171 ± 15	0.0003
C3	54	IgG1	187 ± 30		381 ± 118	0.2667
		IgG2c	1468 ± 405		987 ± 373	0.6634
		IgG3	238 ± 46		275 ± 62	0.2852
		IgM	102 ± 15		155 ± 57	0.4862
C4	54	IgG1	162 ± 57		272 ± 99	0.3465
		IgG2c	350 ± 73		797 ± 162	0.0280
		IgG3	176 ± 52		214 ± 86	0.4118
		IgM	81 ± 9		89 ± 17	>0.999
C5	14	IgG1	139 ± 20	258 ± 37	335 ± 63	0.0172
		IgG2c	240 ± 71	368 ± 73	1201 ± 409	0.0076
		IgG3	89 ± 18	142 ± 19	189 ± 24	0.0132
		IgM	306 ± 68	633 ± 98	875 ± 142	0.0041

^aCongenic fragments according to Figure 4.
^bMale mice with homozygous RIIIS/J alleles in C3 (n = 22), C4 (n = 12) and C5 (n = 10).
^cMale mice with heterozygous alleles in C2 (n = 45) and C5 (n = 10).
^dMale mice with homozygous B10.RIII alleles in C2 (n = 28), C3 (n = 9), C4 (n = 8) and C5 (n = 7).
^eStatistics was calculated with Mann-Whitney U test and Kruskal-Wallis test.
^fArbitrary antibody concentration. Anti-collagen type II antibodies in non-immunised mice are not detectable.
^gMean ± standard error of the mean.

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