Figure 4

Phenotypic characteristics of injected mesenchymal stem cells following 48 hours in culture. Photomicrographs representative of 4',6-diamidino-2-phenylindole (DAPI) counterstaining, green fluorescent protein (GFP) localisation and immunofluorescence (IF) for identical field of view and immunohistochemistry (IHC) in tissue injected with mesenchymal stem cells and cultured for 48 hours post injection: (a) Sox-9, (b) aggrecan (Agg) and (c) type II collagen (Coll II). High Mag IHC, high magnification of indicated IHC region. Scale bar = 570 μm.