Candidate autoantigens identified by mass spectrometry in early rheumatoid arthritis are chaperones and citrullinated glycolytic enzymes

Table 3 Potentially deiminated peptides from MALDI-TOF spectra using the Aldente and FindMod tools

Protein	Sequence of peptides	Theoretical molecular weight (Da)	Position	Missing cleavage
Aldolase	KDGADFAKWR_citrCVLK	1,856.921	139 to 152	3
Phosphoglycerate kinase 1	ALESPER_citrPFLAILGGAK	1,769.979	199 to 215	1
α-Enolase	YNQLLR_citrIEEELGSKAK	1,892.012	406 to 421	2
Calreticulin	DKQDEEQR_citrLK	1,336.623	359 to 368	2
Heat shock protein 60	KDR_citrVTDALNATR	1,360.718	418 to 429	2
	R_citrGVMLAVDAVIAELKK	1,729.988	142 to 157	2
FUSE-BP1	VPDGMVGFIIGR_citrGGEQISR	2,003.038	106 to 124	1
FUSE-BP2	TSMTEEYRVPDGMVGLIIGRGGEQINK (one of these R is citrullinated)	2,967.475	143 to 169	2

FUSE-BP, far-upstream element-binding protein; MALDI-TOF, matrix-assisted laser desorption/ionization–time of flight.

ISSN: 1478-6362