Cyclo-oxygenase 2 (COX-2) expression following co-culture of Candida albicans with synovial fibroblasts. COX-2 expression by synovial fibroblasts was assessed after 12-h co-culture of synovial fibroblasts with different seeding densities of C. albicans. (a) Gene expression. A representative agarose gel demonstrating COX-2 mRNA expression as assessed by reverse transcription polymerase chain reaction (RT-PCR). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) served as internal control. The graph shows the results of densitometric analysis of DNA bands expressed as the mean ± standard deviation (SD) of the relative fold change in COX-2/GAPDH ratio with the ratio of the control condition normalized to 1 (N = 6, * P < 0.05). (b) Protein expression. A representative western blot of COX-2 protein expression following infection by C. albicans with tubulin as an internal protein loading control. The graph shows the results of densitometric analysis of bands expressed as the mean ± SD of the relative change in COX-2/tubulin ratio with the ratio of the control condition normalized to 1 (N = 5, * P < 0.05).