Regulation of catabolic gene expression in normal and degenerate human intervertebral disc cells: implications for the pathogenesis of intervertebral disc degeneration

Table 1 Sequences of primers and probes used in quantitative real-time polymerase chain reaction analysis

Target	Forward primer	Reverse primer	Probe	Accession number
GAPDH	GCTGAACGGGAAGCTCACT	AGGTCAGGTCCACCACTGA	CCCCACTGCCAACGTG	[GenBank: NM_002046]
Aggrecan	CCGTGTGTCCAAGGAGAAGG	GGGTAGTTGGGCAGTGAGAC	CTGATAGGCACTGTTGAC	[GenBank: NM_001135]
Collagen type I	AGAACAGCGTGGCCTACATG	GCGCGGATCTCGATCTCG	CAGCAGACTGGCAAC	[GenBank: NM_000088]
Collagen type II	ATGGAGACTGGCGAGACTTG	GCTGCTCCACCAGTTCTTCTT	CCCAATCCAGCAAACG	[GenBank: NM_001844]
SOX9	GACTTCCGCGACGTGGAC	GTTGGGCGGCAGGTACTG	CGACGTCATCTCCAACAT	[GenBank: NM_000346]
IL-1α	AAGAGGGAAGTTTGCTTGATTAAGG	GAGGATCAAGACTTCTTTGTGCTC	ACCACTGTTCTCTTCTCTACCCTGCCC	[GenBank: NM_000575]
IL-1β	CGGCCACATTTGGTTCTAAGA	AGGGAAGCGGTTGCTCATC	ACCCTCTGTCATTCG	[GenBank: NM_000576]
IL-1 receptor I	ATTTCTGGCTTCTAGTCTGGT	AACGTGCCAGTGTGGAGTGA	ACTTGATTTCAGGTCAATAACGGTCCCC	[GenBank: NM_000877]
IL-1 receptor antagonist	CCTGCAGGGCCAAGCA	GCACCCAACATATACAGCATTCA	AGCCTCGCTCTTGGCAGGTACTCAGT	[GenBank: NM_173841]
TNF-α	CGAACATCCAACCTTCCCAAAC	TGGTGGTCTTGTTGCTTAAAGTTC	CCAATCCCTTTATTACCC	[GenBank: NM_000594]
TNF receptor 1	AATTCTGGCTTCTAGTCTGGT	AACGTGCCAGTGTGGAGTGA	TTCAGTCCCACTCCAGGCTTCACCC	[GenBank: NM_001065]
TNF receptor 2	PDAR	PDAR	PDAR
MMP-3	TGAAGAGTCTTCCAATCCTACTGTTG	CTAGATATTTCTGAACAAGGTTCATGC	TTTGCTCAGCCTATCCAT	[GenBank: NM_002422]
MMP-9	CCCGGAGTGAGTTGAACCA	CAGGACGGGAGCCCTAGTC	TACGTGACCTATGACATC	[GenBank: NM_004994]
MMP-13	CCCCAGGCATCACCATTCAAG	GACAAATCATCTTCATCACCACCAC	CTGCCTTCCTCTTC	[GenBank: NM_002427]

GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IL-1, interleukin-1; MMP, matrix metalloproteinase; PDAR, pre-designed assay; TNF, tumour necrosis factor.

ISSN: 1478-6362