Basal glucose transport and glucose transporter-1 protein in normal and osteoarthritis chondrocytes. (a) 2-Deoxy-D-glucose (2-DG) transport into normal (n = 6) and osteoarthritis (OA) (n = 9) chondrocytes. (b) Concentration dependence of 2-DG influx into normal and OA chondrocytes fitted to the Michaelis–Menten model to determine the affinity and maximal velocity. Each value is the mean ± standard deviation of five independent experiments performed in duplicate. (c) Glucose transporter-1 (GLUT-1) protein normalized to the respective actin band in normal (n = 9) and OA (n = 9) chondrocyte cultures. Bars = mean ± standard deviation.