Stimulation of glucose transport and glucose transporter-1 expression by IL-1β in normal and osteoarthritis chondrocytes. (a) 2-Deoxy-D-glucose (2-DG) transport into normal (n = 4) and osteoarthritis (OA) (n = 5) chondrocytes stimulated or not with IL-1β 30 ng/ml for 48 hours. (b) Glucose transporter-1 (GLUT-1) protein normalized to the respective actin band in normal (n = 4) and OA (n = 9) chondrocyte cultures, stimulated or not with IL-1β 30 ng/ml for 48 hours. Results expressed as the percentage relative to the respective control cells. MW, molecular weight marker. (c) GLUT-1 mRNA levels in normal (n = 3) and OA (n = 3) chondrocyte cultures stimulated or not with IL-1β 30 ng/ml for 12 hours. Results are expressed as the fold increase relative to the respective untreated cells. *P < 0.05 and ***P < 0.001 relative to untreated cells. Bars = mean ± standard deviation.