Table 1 Primer oligonucleotide sequences used for real-time PCR
Gene | Forward primer 5'-3' | Reverse primer 5'-3' | Accession number |
|---|---|---|---|
COL1A1 | CGATGGCTGCACGAGTCACAC | CAGGTTGGGATGGAGGGAGTTTAC | [GenBank:NM_000088] |
COL10A1 | GAACTCCCAGCACGCAGAATCC | GTGTTGGGTAGTGGGCCTTTTATG | [GenBank:NM_000493] |
COL2A1 | CCGGGCAGAGGGCAATAGCAGGTT | CATTGATGGGGAGGCGTGAG | [GenBank:NM_001844] |
COMP | GGGTGGCCGCCTGGGGGTCTT | CTTGCCGCACGCTGATGGGTCTC | [GenBank:NM_000095] |
CRTL1 | GCGTCCGCTACCCCATCTCTA | GCGCTCTAAGGGCACATTCAGTT | [GenBank:NM_001884] |
GAPDH | GGCGATGCTGGCGCTGAGTAC | TGGTTCACACCCATGACGA | [GenBank:NM_000095] |
MMP1 | TACATGCGCACAAATCCCTTCTACC | GAAAAACCGGACTTCATCTCTGTCG | [GenBank:NM_002421] |
MMP13 | CAAAAACGCCAGACAAATGTGACC | GATGCAGGCGCCAGAAGAATCT | [GenBank:NM_002427] |
SOX9 | CTGAGTCATTTGCAGTGTTTTCT | CATGCTTGCATTGTTTTTGTGT | [GenBank:NM_000346] |
TIMP4 | TTTCTTCTGGCTTAGTCTGTTTTCT | ATTCGCCATTTCTCCCCTACCA | [GenBank:NM_003256] |