Cell cycle analysis of FLS. (a) Total cell number. Treatment of advanced glycation end products-modified (AGE)-BSA (5 mg/ml) significantly reduced total cell number after two to seven days in comparison with control-BSA (Co-BSA; *P < 0.001, n = 6). (b) Percentage of cells in the subG1 and G1 phases of the cell cycle. Incubation of fibroblast-like synovial cells (FLS) with AGE-BSA increased the percentage of cells in the subG1 and G1 phases. (*P < 0.01, n = 6). (c) Percentage of cells in the S and G2 phases. AGE-BSA significantly reduced after 16 hours the percentage of cells in the S and G2 phases (*P < 0.01, n = 6).