Table 1 DNA sequences of the sense and antisense primers for real-time PCR analysis and cycler conditions
Gene | Accession number | Primer sequences | Annealing temperature (°C) | Number of cycles | Product size (bp) |
|---|---|---|---|---|---|
GAPDH | [GenBank:J02642] | 5'-CAATGACCCCTTCATTGACC-3' (sense) 5'-TGGACTCCACGACGTACTCA-3' (antisense) | 59 | 30 | 197 |
IL-6 | [GenBank:M14584] | 5'-CTTTTGGAGTTTGAGGTATACCTAG-3' (sense) 5'-CGCAGAATGAGATGAGTTGTC-3' (antisense) | 62 | 30 | 233 |
NFκB p65 | [GenBank:NM_021975] | 5'-AGTACCTGCCAGATACAGACGAT-3' (sense) 5'-GATGGTGCTCAGGGATGACGTA-3' (antisense) | 62 | 30 | 215 |
Osteoprotegerin | [GenBank:U94332] | 5'-TGCAGTACGTCAAGCAGGAG-3' (sense) 5'-CCCATCTGGACATCTTTTGC-3' (antisense) | 53 | 30 | 175 |
p27Kip1 | [GenBank:NM_004064] | 5'-AGATGTCAAACGTGCGAGTG-3' (sense) 5'-TCTCTGCAGTGCTTCTCCAA-3' (antisense) | 59 | 40 | 154 |
RAGE | [GenBank:AB036432] | 5'-GGAAAGGAGACCAAGTCCAA-3' (sense) 5'-CATCCAAGTGCCAGCTAAGA-3' (antisense) | 59 | 30 | 166 |
RANKL | [GenBank:AF019047] | 5'-GCTTGAAGCTCAGCCTTTTG-3' (sense) 5'-CGAAAGCAAATGTTGGCATA-3' (antisense) | 59 | 40 | 192 |
TNF-α | [GenBank:NM_000594] | 5'-GGCAGTCAGATCATCTTCTCGAA-3' (sense) 5'-AAGAGGACCTGGGAGTAGATGA-3' (antisense) | 62 | 40 | 195 |