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Table 1 DNA sequences of the sense and antisense primers for real-time PCR analysis and cycler conditions

From: Advanced glycation end products induce cell cycle arrest and proinflammatory changes in osteoarthritic fibroblast-like synovial cells

Gene Accession number Primer sequences Annealing temperature (°C) Number of cycles Product size (bp)
GAPDH [GenBank:J02642] 5'-CAATGACCCCTTCATTGACC-3' (sense) 5'-TGGACTCCACGACGTACTCA-3' (antisense) 59 30 197
IL-6 [GenBank:M14584] 5'-CTTTTGGAGTTTGAGGTATACCTAG-3' (sense) 5'-CGCAGAATGAGATGAGTTGTC-3' (antisense) 62 30 233
NFκB p65 [GenBank:NM_021975] 5'-AGTACCTGCCAGATACAGACGAT-3' (sense) 5'-GATGGTGCTCAGGGATGACGTA-3' (antisense) 62 30 215
Osteoprotegerin [GenBank:U94332] 5'-TGCAGTACGTCAAGCAGGAG-3' (sense) 5'-CCCATCTGGACATCTTTTGC-3' (antisense) 53 30 175
p27Kip1 [GenBank:NM_004064] 5'-AGATGTCAAACGTGCGAGTG-3' (sense) 5'-TCTCTGCAGTGCTTCTCCAA-3' (antisense) 59 40 154
RAGE [GenBank:AB036432] 5'-GGAAAGGAGACCAAGTCCAA-3' (sense) 5'-CATCCAAGTGCCAGCTAAGA-3' (antisense) 59 30 166
RANKL [GenBank:AF019047] 5'-GCTTGAAGCTCAGCCTTTTG-3' (sense) 5'-CGAAAGCAAATGTTGGCATA-3' (antisense) 59 40 192
TNF-α [GenBank:NM_000594] 5'-GGCAGTCAGATCATCTTCTCGAA-3' (sense) 5'-AAGAGGACCTGGGAGTAGATGA-3' (antisense) 62 40 195
  1. GAPDH = glyceraldehyde 3-phosphate dehydrogenase;
  2. NFκB = nuclear factor kappa B; RAGE = receptor for advanced glycation end products; RANKL = receptor activator of NFκB ligand.