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Figure 3 | Arthritis Research & Therapy

Figure 3

From: Tumor necrosis factor alpha-dependent aggrecan cleavage and release of glycosaminoglycans in the meniscus is mediated by nitrous oxide-independent aggrecanase activity in vitro

Figure 3

Immunostaining of the aggrecan cleavage product NITEGE in paraffin sections of meniscal explants after three days of incubation with or without TNFα, the protease inhibitor TIMP-3 or the NO synthetase inhibitor L-NMMA. There is an increase in NITEGE-staining (green fluorescence) in (d) TNFα-treated samples in comparison to (a, b, c) control tissues, and (e) TIMP-3 is able to inhibit formation of NITEGE (f) in contrast to L-NMMA. Cellular nuclei are counterstained using bisbenzimide (blue fluorescence). L-NMMA = NG-monomethyl-L-arginine.monoacetate; NO = nitric oxide; TIMP = tissue inhibitor of metalloproteinases.

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