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Table 1 List of primers used for real time RT-PCR

From: Tumor necrosis factor alpha-dependent aggrecan cleavage and release of glycosaminoglycans in the meniscus is mediated by nitrous oxide-independent aggrecanase activity in vitro

Target

Sequence (5' to 3')

Product size

GAPDH S

ATC AAG AAG GTG GTG AAG CAG G

101 bp

GAPDH AS

TGA GTG TCG CTG TTG AAG TCG

 

18sRNA S

TCG AGG CCC TGT AAT TGG AA

104 bp

18sRNA AS

GCT ATT GGA GCT GGA ATT ACC G

 

Aggrecan S

CCT GAA CGA CAA GAC CAT CGA

101 bp

Aggrecan AS

TGG CAA AGA AGT TGT CAG GCT

 

Collagen type I S

AAT TCC AAG GCC AAG AAG CAT G

102 bp

Collagen type I AS

GGT AGC CAT TTC CTT GGT GGT T

 

Collagen type II S

AAG AAG GCT CTG CTC ATC CAG G

124 bp

Collagen type II AS

TAG TCT TGC CCC ACT TAC CGG T

 

MMP-1 S

GGA CTG TCC GGA ATG AGG ATC T

91 bp

MMP-1 AS

TTG GAA TGC TCA AGG CCC A

 

MMP-2 S

GTA CGG GAA TGC TGA CGG GGA ATA

93 bp

MMP-2 AS

CCA TCG CTG CGG CCT GTG TCT GT

 

MMP-3 S

CAC TCA ACC GAA CGT GAA GCT

109 bp

MMP-3 AS

CGT ACA GGA ACT GAA TGC CGT

 

MMP-13 S

TCT TGT TGC TGC CCA TGA GT

101 bp

MMP-13 AS

GGC TTT TGC CAG TGT AGG TGT A

 

ADAMTS-4 S

GCG CCC GCT TCA TCA CTG

101 bp

ADAMTS-4 AS

TTG CCG GGG AAG GTC ACG

 

ADAMTS-5 S

AAG CTG CCG GCC GTG GAA GGA A

196 bp

ADAMTS-5 AS

TGG GTT ATT GCA GTG GCG GTA GG

 
  1. ADAMTS = a disintegrin and metalloproteinase with thrombospondin motifs; AS = antisense; bp = base pairs; GAPDH = glyceraldehyde-3-phosphate dehydrogenase; MMP = matrix-metalloproteinase; S = sense.
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Arthritis Research & Therapy

ISSN: 1478-6362

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