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Table 1 List of primers used for real time RT-PCR

From: Tumor necrosis factor alpha-dependent aggrecan cleavage and release of glycosaminoglycans in the meniscus is mediated by nitrous oxide-independent aggrecanase activity in vitro

Target Sequence (5' to 3') Product size
GAPDH S ATC AAG AAG GTG GTG AAG CAG G 101 bp
GAPDH AS TGA GTG TCG CTG TTG AAG TCG  
18sRNA S TCG AGG CCC TGT AAT TGG AA 104 bp
18sRNA AS GCT ATT GGA GCT GGA ATT ACC G  
Aggrecan S CCT GAA CGA CAA GAC CAT CGA 101 bp
Aggrecan AS TGG CAA AGA AGT TGT CAG GCT  
Collagen type I S AAT TCC AAG GCC AAG AAG CAT G 102 bp
Collagen type I AS GGT AGC CAT TTC CTT GGT GGT T  
Collagen type II S AAG AAG GCT CTG CTC ATC CAG G 124 bp
Collagen type II AS TAG TCT TGC CCC ACT TAC CGG T  
MMP-1 S GGA CTG TCC GGA ATG AGG ATC T 91 bp
MMP-1 AS TTG GAA TGC TCA AGG CCC A  
MMP-2 S GTA CGG GAA TGC TGA CGG GGA ATA 93 bp
MMP-2 AS CCA TCG CTG CGG CCT GTG TCT GT  
MMP-3 S CAC TCA ACC GAA CGT GAA GCT 109 bp
MMP-3 AS CGT ACA GGA ACT GAA TGC CGT  
MMP-13 S TCT TGT TGC TGC CCA TGA GT 101 bp
MMP-13 AS GGC TTT TGC CAG TGT AGG TGT A  
ADAMTS-4 S GCG CCC GCT TCA TCA CTG 101 bp
ADAMTS-4 AS TTG CCG GGG AAG GTC ACG  
ADAMTS-5 S AAG CTG CCG GCC GTG GAA GGA A 196 bp
ADAMTS-5 AS TGG GTT ATT GCA GTG GCG GTA GG  
  1. ADAMTS = a disintegrin and metalloproteinase with thrombospondin motifs; AS = antisense; bp = base pairs; GAPDH = glyceraldehyde-3-phosphate dehydrogenase; MMP = matrix-metalloproteinase; S = sense.