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Figure 1 | Arthritis Research & Therapy

Figure 1

From: Hypertrophy is induced during the in vitrochondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer

Figure 1

Transgene expression and biochemical composition of MSCs during 21 days of aggregate culture following BMP-2 and BMP-4 gene transfer. Primary MSCs were infected with Ad.BMP-2, Ad.BMP-4 or Ad.GFP at 5 × 102 vp/cell, seeded into aggregates and analyzed biochemically during a three-week time course. (a, b) Values represent levels of (a) BMP-2 and (b) BMP-4 transgene product expressed in ng/mL in the conditioned media over a 24-hour period at days 3, 7, 14 and 21. At the same time-points cell proliferation was quantified using the (c) WST1 and (d) ATP cell proliferation assay, (e) GAG content and (f) relative ALP activity normalized to DNA is shown. The data represent mean values ± standard deviation from four aggregates per condition and marrow preparation and was performed on five marrow preparations from different patients. Asterisks indicate values that are statistically different (P < 0.05) from marker gene vector-transduced control cultures or between samples. ALP = alkaline phosphatase; ATP = adenosine 5 triphosphate; Ad = adenoviral vector; BMP = bone morphogenetic protein; GAG = glycosaminoglycan; MSC = mesenchymal stem cell.

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Arthritis Research & Therapy

ISSN: 1478-6362

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