Figure 1

Flow cytometry of B-cell subsets in synovial fluid (SF) and peripheral blood (PB) from juvenile idiopathic arthritis (JIA) patients. Mononuclear cells (MNCs) from SF and PB of 25 JIA patients, as well as from PB of 20 age-matched controls, were stained with different B cell-specific antibodies and analyzed with flow cytometry. The first gate was set on CD3^-, CD14^-, CD16^-, and CD56^- cells (non-B cell lineage cells) followed by gating on CD19⁺ cells. Results are expressed in a box plot (left panel) as median percentage of positive cells, minimum and maximum value. * P < 0.01. One representative dot-blot for SF (middle panel) and PB (right panel) is shown.