Figure 2

Transitional B cells in synovial fluid (SF) and peripheral blood (PB) in juvenile idiopathic arthritis (JIA) patients. Cells from SF and PB of JIA patients, as well as from control PB, were analyzed with flow cytometry by gating on CD3^-, CD14^-, CD16^-, and CD56^- cells (non-B cell lineage cells) followed by gating on CD19⁺ cells and then on CD24^high, CD38^high cells. Results are expressed in a box plot (left panel) as median percentage of positive cells, minimum and maximum value. *P < 0.01 (patient PB versus SF) and P = 0.05 (patient versus control PB). One representative dot-blot for SF (middle panel) and PB (right panel) is shown. Insets on the right side of the Figure show that gated CD24^highand CD38^high B cells express immunoglobulin M and immunoglobulin D (upper inset) and CD10 (lower inset).