Figure 3

Regulation of IL-17 responses by Th2 cytokine IL-4 during the initiation phase of arthritis. (a) Neutralizing antibody to IFN-γ (clone R46A2, 100 μg/mouse/day), and/or neutralizing antibody to IL-4 (clone 11B11, 100 μg/mouse/day) was administered intraperitoneally from day 10 to 20 after immunization with collagen and complete Freund's adjuvant (CFA). Rat IgG was used as isotype control. The control group did not receive any antibody. Arthritis was assessed from day 10 by clinical scoring. Data is representative of two experiments, with n = 8 in each group. * P < 0.05. (b) Sera from day 22 after collagen immunization were analyzed for IL-17, IL-4, and IFN-γ by ELISA. Data is presented as mean +/- standard error of the mean (SEM). Data is representative of two experiments, with n = 8 in each group. (c) The paws of mice from the different groups were cultured in media overnight. The supernatants were analyzed for IL-17, IL-4 and IFN-γ by ELISA. Data presented as mean +/- SEM. Data is representative of two experiments, with n = 8 in each group.* P < 0.05. (d) The paws of mice from the different groups were cultured in media overnight. The supernatants were analyzed for IL-10 by ELISA. Data presented as mean +/- SEM. * P < 0.01.