Figure 4

Specimen Calc7. (a and b) Images of the top of the specimen as received and after freeze drying, respectively. (c to f) X-ray micro-computed tomography (μCT) 3D projection image, visual and Fourier Transform Infrared microspectroscopy in reflectance mode (FTIR-RM) protein and mineral maps of the top, respectively, after embedding and polishing. * indicates the same exposed surfaces in the 3D μCT image (c) and the visual map (d). (g and h) Images of the cut cross section of the specimen as received and after freeze drying, respectively. (i to l) μCT 3D projection image, visual and FTIR-RM protein and mineral maps of the cut cross section, respectively, after embedding and polishing. The distance between the (d) polished top and the (j) polished cut cross section is 3.4 mm. All FTIR-RM maps were obtained with 180 × 180 μm spatial resolution (SR). Blue is the largest area under the related peaks and red is the lowest in the FTIR-RM maps. Blue arrows in G and K point to the surrounding tissue. The individual size scale bars appear on the images and the maps.