|
Sample/Analysis
| | | | | | | | | |
|---|
|
qBSE
|
MD, g/ml
|
|
Enamel
|
1: 2.98 ± 0.10;
|
2: 2.99 ± 0.09
|
|
Dentin
|
1: 2.46 ± 0.44;
|
2: 2.46 ± 0.32
|
|
Calcified cartilage
|
1: 2.36 ± 0.67;
|
2: 2.50 ± 0.81
|
|
Bone
|
1: 2.14 ± 0.55;
|
2: 2.19 ± 0.55
|
|
Calc1
|
W: 2.65 ± 0.74
| | | | |
|
Calc2
|
W: 2.51 ± 0.74
| | | | |
|
Calc2
|
CE 1: 2.68 ± 0.55;
|
2: 2.53 ± 0.75
|
|
Calc2
|
3: 2.23 ± 0.79;
|
4*: 2.58 ± 0.54;
|
| |
5: 2.66 ± 0.60
| | | | |
|
Calc3**
|
W: 2.52 ± 0.92 (377);
|
HC: 2.69 ± 0.11 (245)
|
|
Calc4**
|
W: 2.400 ± 1.09 (325);
|
HC: 2.66 ± 0.21 (147)
|
|
FTIR-RM
|
PO
4
/Am I
|
AmI/PO
4
|
Calc/Bone
| | |
| | | |
%
|
%
| | |
|
Dentin, Fig 7a
|
10.5
|
9.6
| | | | |
|
Bone, Fig 7a
|
7.4
|
13.5
| | | | |
|
Calc1, High Prot
|
11.8
|
8.5
|
62.7
| | |
|
Calc1, Fig 7a
|
19.4
|
5.2
|
38.1
| | |
|
Calc2, Fig 7a
|
27.8
|
3.6
|
26.6
| | |
|
Calc10, Fig 7a
|
51.0
|
2.0
|
14.5
| | |
|
Calc7, Fig 7a
|
30.6
|
3.3
|
24.2
| | |
|
Calc8, Fig 7a
|
162.3
|
0.6
|
4.6
| | |
|
SEM/SDD-EDS
|
Ca
|
P
|
Ca/P
|
Mg
|
Na
|
Cl
|
S
|
Al
|
| | |
%
|
%
|
MR
|
%
|
%
|
%
|
%
|
%
|
|
Calc1-1:
|
Average
|
39.4
|
18.3
|
1.66
|
0.60
|
1.28
|
0.07
|
0.01
|
0.07
|
| |
SD
|
0.02
|
0.02
| |
0.01
|
0.01
|
0.01
|
0.005
|
0.01
|
|
Calc1-2:
|
Average
|
39.8
|
18.2
|
1.69
|
0.62
|
1.07
|
0.05
|
0.02
|
0.07
|
| |
SD
|
0.02
|
0.02
| |
0.01
|
0.01
|
0.01
|
0.005
|
0.01
|
|
Calc2:
|
Average
|
39.1
|
18.5
|
1.63
|
0.53
|
1.16
|
0.11
|
0.06
|
0.06
|
| |
SD
|
0.02
|
0.02
| |
0.01
|
0.01
|
0.01
|
0.01
|
0.01
|
|
XRD
|
1/FWHM
|
Crystallinity (Relative to Bone)
|
|
Phys Apatite
|
2.50
|
1.30
|
|
Dentin
|
1.99
|
1.03
|
|
Bone
|
1.93
| | | | |
|
Calc7-Powder
|
3.76
|
1.95
|
|
Calc7-Polished
|
4.10
|
2.13
|
|
Calc8-Polished
|
4.85
|
2.52
|
|
Enamel
|
3.53
|
1.83
|
|
OHAp
|
5.95
|
3.09
|
- W = whole image; CE = calcified element; * Figure 5f; ** qBSE images in Figure 7c; HC = highly calcified regions (> 2.60 g/ml).
- n = 1024 pixels for qBSE analyses of all calcified tissues and Calc1 and Calc2 images.
- % = weight %; MR = molar ratio.
- FTIR-RM = Fourier Transform Infrared microspectroscopy in reflectance mode; FWHM = full width at one half the maximum height; qBSE = quantitative backscattered electron; SEM/SDD-EDS = scanning electron microscopy/silicon drift detector energy dispersive X-ray spectrometry; XRD = X-ray diffraction.
