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Table 2 qBSE, FTIR-RM, SEM/SDD-EDS and XRD results obtained from various calcified tissues, calcinosis samples and synthetic apatites

From: Microstructure and mineral composition of dystrophic calcification associated with the idiopathic inflammatory myopathies

Sample/Analysis          
qBSE MD, g/ml
Enamel 1: 2.98 ± 0.10; 2: 2.99 ± 0.09
Dentin 1: 2.46 ± 0.44; 2: 2.46 ± 0.32
Calcified cartilage 1: 2.36 ± 0.67; 2: 2.50 ± 0.81
Bone 1: 2.14 ± 0.55; 2: 2.19 ± 0.55
Calc1 W: 2.65 ± 0.74     
Calc2 W: 2.51 ± 0.74     
Calc2 CE 1: 2.68 ± 0.55; 2: 2.53 ± 0.75
Calc2 3: 2.23 ± 0.79; 4*: 2.58 ± 0.54;
  5: 2.66 ± 0.60     
Calc3** W: 2.52 ± 0.92 (377); HC: 2.69 ± 0.11 (245)
Calc4** W: 2.400 ± 1.09 (325); HC: 2.66 ± 0.21 (147)
FTIR-RM PO 4 /Am I AmI/PO 4 Calc/Bone   
    % %   
Dentin, Fig 7a 10.5 9.6     
Bone, Fig 7a 7.4 13.5     
Calc1, High Prot 11.8 8.5 62.7   
Calc1, Fig 7a 19.4 5.2 38.1   
Calc2, Fig 7a 27.8 3.6 26.6   
Calc10, Fig 7a 51.0 2.0 14.5   
Calc7, Fig 7a 30.6 3.3 24.2   
Calc8, Fig 7a 162.3 0.6 4.6   
SEM/SDD-EDS Ca P Ca/P Mg Na Cl S Al
   % % MR % % % % %
Calc1-1: Average 39.4 18.3 1.66 0.60 1.28 0.07 0.01 0.07
  SD 0.02 0.02   0.01 0.01 0.01 0.005 0.01
Calc1-2: Average 39.8 18.2 1.69 0.62 1.07 0.05 0.02 0.07
  SD 0.02 0.02   0.01 0.01 0.01 0.005 0.01
Calc2: Average 39.1 18.5 1.63 0.53 1.16 0.11 0.06 0.06
  SD 0.02 0.02   0.01 0.01 0.01 0.01 0.01
XRD 1/FWHM Crystallinity (Relative to Bone)
Phys Apatite 2.50 1.30
Dentin 1.99 1.03
Bone 1.93     
Calc7-Powder 3.76 1.95
Calc7-Polished 4.10 2.13
Calc8-Polished 4.85 2.52
Enamel 3.53 1.83
OHAp 5.95 3.09
  1. W = whole image; CE = calcified element; * Figure 5f; ** qBSE images in Figure 7c; HC = highly calcified regions (> 2.60 g/ml).
  2. n = 1024 pixels for qBSE analyses of all calcified tissues and Calc1 and Calc2 images.
  3. % = weight %; MR = molar ratio.
  4. FTIR-RM = Fourier Transform Infrared microspectroscopy in reflectance mode; FWHM = full width at one half the maximum height; qBSE = quantitative backscattered electron; SEM/SDD-EDS = scanning electron microscopy/silicon drift detector energy dispersive X-ray spectrometry; XRD = X-ray diffraction.