Figure 1From: Effect of small interference RNA (siRNA) for ADAMTS5on intervertebral disc degeneration in the rabbit anular needle-puncture modelEstablishment of small interfering RNA (siRNA) oligonucleotide for ADAMTS5 in rabbit nucleus pulposus (NP) cells. After the 48-hour preculture period, rabbit NP cells were transfected with the siRNA oligonucleotide specific for either the control or ADAMTS5. At 48 hours after transfection in NP cells, the ADAMTS5 siRNA-transfected cells showed approximately a 75% knock-down of ADAMTS5 mRNA compared with the control siRNA. The results are reported normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH).Back to article page