Figure 3
Effect of ADAMTS5 oligonucleotide on rabbit nucleus pulposus (NP) cells in alginate bead culture. NP cells were suspended in sodium alginate at a density of 5 × 105 cells/ml and maintained for up to 14 days. After 14 days, the NP cells in alginate beads were transfected with anti-ADAMTS5 oligonucleotide or control oligonucleotide without using gene-delivery reagents. In NP cells in alginate bead culture, the expression of the ADAMTS5 gene was approximately 70% of that seen with the control oligonucleotide. The results are reported normalized to GAPDH.