Table 2 Sensitivity of PCR for the detection of Chlamydia trachomatis in synovial fluid depending on DNA extraction method and primer system used
Amplification system | 1 Alkaline Lysis | 2 Qiaex II gel extraction kit® | 3 Chelex® | 4 Qiagen Tissue kit® | 5 Qiagen Stool kit® |
|---|---|---|---|---|---|
(C. trachomatisEB/ml SF) | (C. trachomatisEB/ml SF) | (C. trachomatisEB/ml SF) | (C. trachomatisEB/ml SF) | (C. trachomatisEB/ml SF) | |
PCR 1 | 1 | 0.1 | 100 | 1 | 1 |
C. trachomatis | (0.1-10) | (1-100) | (10-100) | (1-10) | (1-100) |
omp 1 | M1 | M 1 | M 100 | M 1 | M 100 |
Statistical analysis | * 2,5 | * 1,4 | * 2 | * 1 | |
PCR 2 | 10 | 1000 | 1000 | 10 | 10 |
C. trachomatis | (10) | (1-1000) | (100-1000) | (0.1-10) | (10-1000) |
omp 1 | M 10 | M 1000 | M 1000 | M10 | M 10 |
Statistical analysis | * 2 | * 1,4,5 | * 2 | * 2 | |
PCR 3 | 1 | 100 | 1000 | 1 | 100 |
Plasmid | (0.1-10) | (10-1000) | (1000) | (1-10) | (10-1000) |
M 1 | M 100 | M 1000 | M 1 | M 100 | |
Statistical analysis | * 2,3,5 | * 1,3,4,5 | * 1,2 | * 2,3 | * 1,2,4 |
PCR 4 | 1 | 10 | 1000 | 10 | 100 |
16 sRNA | (0.1-10) | (10-1000) | (10-1000) | (0.1-10) | (10-100) |
M 1 | M 100 | M 1000 | M 10 | M 10 | |
Statistical analysis | * 2,3,5 | * 1,4,5 | * 1 | * 2,3 | * 1,2 |
LCR | 10 | 1 | 10000 | 10 | 100 |
Plasmid | (0.1-100) | (1-100) | (100-1000) | (10- 100) | (1-1000) |
M 10 | M 100 | M 1000 | M 10 | M 100 | |
Statistical analysis | * 2 | * 1,3,4,5 | * 2 | * 2 | * 2 |