Figure 4From: A combination of methotrexate and zoledronic acid prevents bone erosions and systemic bone mass loss in collagen induced arthritisRepresentative TRAP staining profiles. The (a) control, (b) zoledronic acid (ZA), (c) methotrexate (MTX), and (d) ZA + MTX groups are shown (×40). (e) Erosion score. Bone erosion was strongly inhibited in the ZA group. MTX also significantly reduced bone erosion, although to a lesser extent than ZA. (f) Number of osteoclasts (multinucleated tartrate resistant acid phosphatase (TRAP) positive cells) at the bone/synovium interface in the eroded joint of the tibiotarsal and intertarsal joints. ZA, used alone or in combination with MTX, strongly decreased the number of TRAP-positive cells. MTX also decreased the number of TRAP+ cells in the eroded joint. *P < 0.05 versus computed tomography (CT) group; **P < 0.005 versus CT group; +P < 0.05 versus MTX group.Back to article page