Focus score and infiltrate analysis in untreated and vector treated groups. A) Salivary glands were removed for histologic analysis at 24 weeks, fixed and stained with Hematoxylin and eosin. Histopathologic assessment was performed and presented as a focus score (see Materials and Methods). Data shown are the mean (+/- SD). No significant change was detected by one-way ANOVA test followed by unpaired student's t-test. Untreated (N = 7), LacZ (N = 8) and TNFR1:IgG (N = 6) mice. B) Acetone fixed frozen sections from SGs were analyzed for CD4, CD8, CD19 and CD138 by immunohistochemistry followed by digital analysis as described in the Material and Methods section. Data shown are the mean positive cell counts per mm2 (+/- SEM). Higher means were observed for CD4, CD8 and CD138 in the TNFR1:IgG treated mice, but no significant changes were detected by non-parametric Wilcoxon's ranksum test. LacZ (N = 4) and TNFR1:IgG (N = 8).