Effect of Rac1 carboxy-terminal peptide treatment of mice on lymphocyte numbers and percentages in blood and spleen. Mice treated with Ctrl or Rac1 peptide (2 mg, n = 5 per group) were sacrificed on day 28 following arthritis induction, and CD3+ T cells and B220+ B cells were identified by fluorescence-activated cell sorting analysis in (a) blood and (b) spleen. Numbers of CD3+ T cells (upper panels) and B220+ cells (lower panels) were calculated as percentage positive cells (left panels) or total number of cells (right panels) per milliliter of blood or per spleen. Data points within each column represent individual mice, and the bar indicates the median value. Ctrl, control.