Figure 4

Attenuated disease development by CD40 siRNA. DBA mice were immunized with 200 μg CII antigen, two times on Day 0 and Day 21, respectively. Two days after CII immunization, the mice were treated with 50 μg of CD40 siRNA using the hydrodynamic injection method. siRNA treatment was repeated two weeks later. Disease severity was scored from the day of last immunization to five weeks a). The swelling of joints of CIA mice was determined by the thickness of each hind paw measured with caliper b). Data are presented as mean ± SEM. Results represent one of three experiments. * = P < 0.05 versus control siRNA.