Figure 5

CHOP plays an important role in autophagy in RASF and in cell death in OASF. OASF and RASF were treated with thapsigargin (5 μM) or tunicamycin (5 μg/ml) for 60 h. Autophagy formation was measured as described in methods *P < 0.05, significantly different from thapsigargin-treated OASF, ^#P < 0.05, significantly different from tunicamycin-treated OASF (a). Non-specific or CHOP siRNA was transfected into OASF and RASF. After 16 h, cells were treated with thapsigargin (5 μM) or tunicamycin (5 μg/ml) for 4 h. Immunoblotting was performed with anti-CHOP or actin antibody (b). Autophagy formation (c) and cell death (d) were measured. *P < 0.05, significantly different from non-specific siRNA-transfected OASF in the presence of thapsigargin. ^#P < 0.05, significantly different from non-specific siRNA-transfected OASF in the presence of tunicamycin, ^$P < 0.05, significantly different from non-specific siRNA-transfected RASF in the presence of thapsigargin. ^&P < 0.05, significantly different from non-specific siRNA-transfected RASF in the presence of tunicamycin.