Table 1 Bovine oligonucleotide primers
Gene name | Gene symbol | NCBI ref. seq. | Forward primer | Reverse primer |
|---|---|---|---|---|
Glyceraldehyde-3-phosphate dehydrogenase | GAPDH | NM_001034034.1 | TGCCGCCTGGAGAAACC | CGCCTGCTTCACCACCTT |
Aggrecan | ACAN | NM_173981.2 | GGGAGGAGACGACTGCAATC | CCCATTCCGTCTTGTTTTCTG |
Versican | VCAN | NM_181035.2 | GCTGCATGCCGCCTATG | TCCGTAGGTCCGGACTCCTT |
Collagen, type II, alpha 1 | COL2A1 | NM_001001135.2 | CGGGCTGAGGGCAACA | CGTGCAGCCATCCTTCAGA |
Synaptosomal associated protein 25 | SNAP25 | NM_001076246.1 | GGCTTCATCCGCAGGGTAA | GCTCCAGGTTTTCATCCATTTC |
Keratin 8 | KRT8 | NM_001033610.1 | ACCAGGAGCTCATGAATGTCAA | TCGCCCTCCAGCAGCTT |
Keratin 18 | KRT18 | XM_582930.4 | TTGAGCTGCTCCATCTGCAT | AAGGCCAGCTTGGAGAACAG |
Keratin 19 | KRT19 | XM_875997.3 | CGGTGCCACCATTGAGAACT | CAAACTTGGTGCGGAAGTCA |
N-Cadherin | CDH2 | XM_001250829.2 | GCCATCAAGCCAGTTGGAA | TGCAGATCGAACCGGGTACT |
Sclerostin domain containing 1 | SOSTDC1 | NM_001046265.1 | GTTCAAGTAGGCTGCCGAGAA | GCACTGGCCGTCTGAGATG |
Integrin-binding sialoprotein | IBSP | NM_174084.2 | GACAGCTATGATGGTCAAGATTACTACA | TGGGTGAACTCATCCCAGTCT |
Tenomodulin | TNMD | NM_001099948.1 | TCTGGCGTGACGGGTCTT | AAAAAAGGCATTGAACAAAACGA |
Brain attached signalling protein 1 | BASP1 | NM_174780.3 | TTGTGGATGAATGCCAACTTTC | AAAAATGGAGTATTGGCATCAAGAT |
TNF, alpha-induced protein 6 | TNFAIP6 | NM_001007813.1 | AAGCAGCAGGCGTCTACCA | CACACCGCCTTCGCTTCT |
Forkhead box F1 | FOXF1 | XM_603148.4 | TCCCTCCCCACCTCAGAAGT | TGGCTTCAGAAATGCAAGTTACTC |
Forkhead box F2 | FOXF2 | CK941878.1 | TGCGTGGTAAGTTTTCACCATCT | CCCCCGGTGAGGTAATGC |
Aquaporin 1 | AQP1 | NM_174702.3 | ACCAGGAGGCCCTTAATGGT | CTGTTAAATGGACTAGAAGCGAAATG |
Fibulin 1 | FBLN1 | NM_001098029.1 | GCAGCGCAGCCAAGTCAT | AGATATGTCTGGGTGCTACAAACG |
T, brachyury homolog (mouse) | T | XM_864890.2 | ACTTCGTGGCGGCTGACA | GCACCCACTCCCCATTCA |