Figure 2

Effect of exogenous IL-17 on type I collagen metabolism in RA synovium explants. Synovium samples from RA patients were incubated for 7 days in the presence of IL-17 (50 ng/ml) or IL-1 (100 pg/ml). (a) PICP (n = 4) levels in supernatants were measured by ELISA. Results are expressed as mean ± SEM of % induction of PICP production. Spontaneous production of PICP was 371 ± 36 ng/ml. (b) CTX levels (n = 7) in supernatants were measured by ELISA. Results are expressed as mean ± SEM. Spontaneous production of CTX was 33 ± 11 ng/ml. ^*P < 0.05, ^**P < 0.01 compared with control (medium alone).