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Table 1 The oligonucleotide sequences of primers

From: B lymphocyte involvement in ankylosing spondylitis: the heavy chain variable segment gene repertoire of B lymphocytes from germinal center-like foci in the synovial membrane indicates antigen selection

Oligonucleotides (2.5 mM)
JH1 5’ CGTCGTCGAC CAGGGTGCCC TGGCCCCAGT GC (101–109)
JH2 5’ CGTCGTCGAC CAGGGTGCCC TGGCCCCAGT GC (101–109)
JH3 5’ CGTCGTCGAC ATTGTCCCTT GGCCCCAGAC ATCA (100–108)
JH4 5’ CGTCGTCAAC CACGGTTCCT TGGCCCCAGT AG (101–109)
JH5 5’ CGTCGTCGAC GTGACCAGGG TTCCTTGGCC CCAGG (102–110)
JH6 5’ CGTCGTCGAC GTGGTCCCTT GCCCCCAGAC GTCC (100–108)
VH1 5’ CTACGTCGAC CCTCAGTGAA GGTYTCCTGC AAGGC (16–24)
VH2 5’ GCACGTCGAC GTCCTGCGCT GGTGAAASCC ACACA (16–24)
VH3 5’ GTACGTCGAC GGGGTCCCTG AGCTCTCCTG TGCAG (15–24)
VH4 5’ CGTCGTCGAC CCTGTCCCTC ACCTGCRCTG TC (16–24)
VH5 5’ CGACGTCGAC AAAAAGCCCG GGGAGTCTCT GARGA (12–20)
VH6 5’ CGTCGTCGAC CTGTGCCATC TCCGGGGACA GTG (21–29)
JH Intron 1,2-4,5 5’GACTCACCTG AGGAGACGGT GACC
JH Intron 3,6 5’TCTTGCCTGA GGAGACGGTG ACCRT
  1. All oligonucleotides contain a 5¢ SalI restriction site (G/TCGAC). The corresponding amino acid positions are presented in parentheses. The VH1 oligonucleotide also perfectly matches VH7.