Table 1 The oligonucleotide sequences of primers

JH1 5’ CGTCGTCGAC CAGGGTGCCC TGGCCCCAGT GC (101–109)

JH2 5’ CGTCGTCGAC CAGGGTGCCC TGGCCCCAGT GC (101–109)

JH3 5’ CGTCGTCGAC ATTGTCCCTT GGCCCCAGAC ATCA (100–108)

JH4 5’ CGTCGTCAAC CACGGTTCCT TGGCCCCAGT AG (101–109)

JH5 5’ CGTCGTCGAC GTGACCAGGG TTCCTTGGCC CCAGG (102–110)

JH6 5’ CGTCGTCGAC GTGGTCCCTT GCCCCCAGAC GTCC (100–108)

VH1 5’ CTACGTCGAC CCTCAGTGAA GGTYTCCTGC AAGGC (16–24)

VH2 5’ GCACGTCGAC GTCCTGCGCT GGTGAAASCC ACACA (16–24)

VH3 5’ GTACGTCGAC GGGGTCCCTG AGCTCTCCTG TGCAG (15–24)

VH4 5’ CGTCGTCGAC CCTGTCCCTC ACCTGCRCTG TC (16–24)

VH5 5’ CGACGTCGAC AAAAAGCCCG GGGAGTCTCT GARGA (12–20)

VH6 5’ CGTCGTCGAC CTGTGCCATC TCCGGGGACA GTG (21–29)

JH Intron 1,2-4,5 5’GACTCACCTG AGGAGACGGT GACC

JH Intron 3,6 5’TCTTGCCTGA GGAGACGGTG ACCRT