Figure 6

BAY11 inhibits in vitro pathogenic conditioned IFN-α production. (A) PBMCs from three patients with SLE were isolated and 1 × 10⁶ cells were preincubated for 15 minutes with BAY11 (10^-9 to 10^-5 M) or vehicle in the presence of the autologous serum (at a final concentration of 20% vol/vol) in 500 μl of medium per well with 5 μM CpG 2216 for 24 hours. Data are shown as mean ± SEM of three independent experiments. Statistical significance was determined using paired Student's t test (*P < 0.05 **P < 0.01). (B) Human healthy PBMCs were preincubated for 15 minutes with BAY11 (10^-9 to 10^-7 M) or vehicle in the serum-free RPMI, and the serum of a SLE patient (at a final concentration of 20% vol/vol) with or without necrotic cell supernatant (at a final concentration of 20% vol/vol) was then added. After 24 hours, the concentrations of IFN-α in the culture supernatants were measured by ELISA. Data are shown as mean ± SEM of four independent donors. Statistical significance was determined using Mann-Whitney test (*P < 0.05 **P < 0.01).