Comparative analysis of blood CD27 IgD IgM B cell-subsets from healthy controls and different groups of SLE patients according to the presence of autoantibodies. A. B cell subsets from healthy controls (n = 31) and SLE patients with negative or low levels (IIF titre ≤1/160; n = 23) and medium or high levels (IFF titre ≥1/320; n = 46) of ANA were compared. B and C. Comparison among healthy controls and SLE patients with anti-dsDNA negative (n = 46) or positive (n = 23) and anti-ENA negative (n = 41) or positive (n = 28) Ab, respectively. Differences between groups are indicated (* P < 0.05; ** P < 0.01).