Figure 3

Expression level of CD163 and CD204 in CD14⁺ PBMCs. (a) Left panels (healthy controls) and middle panels (systemic sclerosis (SSc) patients) depict staining with each isotype-matched monoclonal antibody control (Iso) (unfilled graph) and antigen-specific monoclonal antibody indicated (Test) (filled graph). Right panels depict staining with each monoclonal antibody in healthy controls (Test C) (fine line) versus SSc patients (Test SSc) (bold line). Data presented here are representative of 16 healthy controls and 15 (for CD163) or 41 (for CD204) SSc patients. (b) Comparison of expression level of CD163 and CD204 in CD14⁺ peripheral blood mononuclear cells (PBMCs) between healthy controls and SSc patients. Mean fluorescence intensity (MFI) are shown on the ordinate. *P < 0.05 as compared with the value in cells from healthy controls. (c) The left panel shows the representative pattern of CD204 histogram. The number of peaks was counted as 1 in the (i) left panel, (ii) 1.5 in the middle panel and (iii) 2 in the right panel. The right panel shows increased number of peaks in CD204 histogram in SSc patients. The number of peaks is shown on the ordinate. * P < 0.05 as compared with the value in cells from healthy controls.