Figure 2

Effect of IL-27 on the cell surface expression of adhesion molecules on control and RA-FLS. (A, C). Control and RA-FLS were cultured with IL-27 (50 ng/ml) for 48 h and then the surface expression of (A) ICAM-1 and (C) VCAM-1 was analysed by flow cytometry. The modulation of surface expression of adhesion molecules are shown in bar chart as MFI. Results have been normalized by subtracting appropriate isotypic control and are expressed as the arithmetic mean plus SD of three independent experiments. Representative histograms of cell surface expression of (A) ICAM-1 and (C) VCAM-1 on control FLS and RA-FLS were determined by flow cytometry. (B, D) Control and RA-FLS were stimulated with IL-27 (0 to 100 ng/ml) for 12 to 48 h and then the surface expression of (B) ICAM-1 and (D) VCAM-1 was analysed by flow cytometry. Results have been normalized by subtracting appropriate isotypic control and are expressed as the arithmetic mean SD of MFI from triplicate experiments. CTL: control, **P < 0.01, ***P < 0.001.