Effects of IL-27 on intracellular STAT-1, JAK-2, AKT, JNK and PI-3K activities in FLS. Control or RA-FLS were incubated with IL-27 (50 ng/ml) from 0 to 30 minutes. The amounts of intracellular phosphorylated signaling molecules in 5,000 permeabilized cells were measured by flow cytometry. Results of (A) phosphorylated STAT-1, (B) phosphorylated JAK-2, (C) phosphorylated AKT, (D) phosporylated PI3K and (E) phosphorylated JNK are shown in MFI subtracting corresponding isotypic control and are expressed as the arithmetic mean plus SD of three independent experiments. Representative histograms illustrate the intracellular expression of (A) phosphorylated STAT-1, (B) phosphorylated JAK-2, (C) phoshorylated AKT, (D) phosphorylated PI3K and (E) phosphorylated JNK in control or RA-FLS. The isotypic control represents the cell populations stained with anti-mouse IgG1 isotype control. *P < 0.05, **P < 0.01 and ***P < 0.001 when compared between groups denoted by horizontal lines.