Treatment with eicosapentaenoic acid (EPA) reduced the rate of cartilage degradation in the presence of IL-1β. Over the 120 treatment period, sGAG release was measured in cartilage explants treated with EPA in the presence (a) and absence (b) of IL-1β. EPA was used at 0.1, 1 and 10 μM. Median data are shown with interquartile range error bars. Both sub-figures are on the same scale for comparative purposes. Statistically significant differences are shown relative to the untreated control group (a) and the IL-1β treatment group (b). Analysis was conducted using Mann-Whitney U tests (*P < 0.05, **P < 0.01, ***P < 0.001).